FITC-labeled Anti-SAP can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-SAP to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line.
Saporin was used as the immunogen. The antibody was affinity-purified against saporin attached to a CnBr-Sepharose support column. The affinity-purified polyclonal antiserum was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP. The conjugate binds native and recombinant saporin.
Applications include flow cytometry.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols
Advanced Targeting Systems(ATS)位于美国加州圣地亚哥, 成立于1994年。 公司发起人Douglas Lappi和Ronald Wiley博士在实验室开发出先进的分子神经外科学研究技术。ATS主要生产用于科研和药物研发的靶向试剂,公司在疼痛和药物转运的研究及临床治疗的两个产品已获专利保护。目前,产品包括靶向毒素 (可提供基底前脑胆碱能神经元、去甲肾上腺素能和肾上腺素能神经元、巨噬细胞和小胶质细胞的特异性损伤试剂等)、二抗生物素、神经元抗体、神经递质抗体、蛋白质和荧光结合物、免疫毒素对照等。