A fusion tag called FLAG consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M5 detects N-terminal Met-FLAG fusion proteins.

Anti-FLAG (M5)-ZAP uses the secondary antibody (antibody to N-Terminal Met-FLAG fusion protein) to “piggyback” onto YOUR FLAG-tagged recombinant proteins or FLAG-tagged proteins over-expressed in cells to evaluate the expression of your target. Once the conjugate is internalized, saporin breaks away from the targeting agent and inactivates the ribosomes, which causes protein inhibition and, ultimately, cell death.

Anti-FLAG (M5)-ZAP specifically eliminates YOUR FLAG-tagged recombinant proteins or FLAG-tagged proteins over-expressed in cells.

To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols

Advanced Targeting Systems(ATS)位于美国加州圣地亚哥， 成立于1994年。 公司发起人Douglas Lappi和Ronald Wiley博士在实验室开发出先进的分子神经外科学研究技术。ATS主要生产用于科研和药物研发的靶向试剂，公司在疼痛和药物转运的研究及临床治疗的两个产品已获专利保护。目前，产品包括靶向毒素 (可提供基底前脑胆碱能神经元、去甲肾上腺素能和肾上腺素能神经元、巨噬细胞和小胶质细胞的特异性损伤试剂等)、二抗生物素、神经元抗体、神经递质抗体、蛋白质和荧光结合物、免疫毒素对照等。