Streptavidin-pHast is one of our fastest tools for quantitative testing of your biotinylated protein’s specificity, binding, and internalization, providing results in 1 day. Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast). This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell.
This secondary conjugate recognizes YOUR biotinylated protein. Streptavidin-pHast is a conjugate of streptavidin and a pH-dependent fluorescent reporter. Approximately 17 micrograms of Streptavidin-pHast is enough to test 1 biotinylated protein (6 replicates at 8 different concentrations) with a starting concentration of 100 nM in a 96-well plate.
View protocol(s) for this and other pHast products.
Advanced Targeting Systems(ATS)位于美国加州圣地亚哥, 成立于1994年。 公司发起人Douglas Lappi和Ronald Wiley博士在实验室开发出先进的分子神经外科学研究技术。ATS主要生产用于科研和药物研发的靶向试剂,公司在疼痛和药物转运的研究及临床治疗的两个产品已获专利保护。目前,产品包括靶向毒素 (可提供基底前脑胆碱能神经元、去甲肾上腺素能和肾上腺素能神经元、巨噬细胞和小胶质细胞的特异性损伤试剂等)、二抗生物素、神经元抗体、神经递质抗体、蛋白质和荧光结合物、免疫毒素对照等。