FITC-labeled goat anti-mouse IgG can be used to verify specific binding of mouse IgG to its receptor. By first incubating cells with the primary mouse antibody, and then binding the FITC-labeled goat anti-mouse IgG to the primary antibody, a fluorescent marker is formed that can demonstrate expression of a receptor or affinity of an antibody for its receptor. FITC is excited by 488 nm wavelength light, and emits at 525 nm.

This fluorescent conjugate was prepared using goat anti-mouse IgG (H+L) and the fluorescent compound, fluorescein isothiocyanate (FITC). The antibody binds to mouse IgG, and is affinity-purified to decrease background and non-specific binding. This antibody exhibits maximal binding to mouse IgG antibodies, and minimal cross-reactivity with other molecules. This product is routinely tested by flow cytometry.

Applications include flow cytometry.

To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols

Advanced Targeting Systems(ATS)位于美国加州圣地亚哥， 成立于1994年。 公司发起人Douglas Lappi和Ronald Wiley博士在实验室开发出先进的分子神经外科学研究技术。ATS主要生产用于科研和药物研发的靶向试剂，公司在疼痛和药物转运的研究及临床治疗的两个产品已获专利保护。目前，产品包括靶向毒素 (可提供基底前脑胆碱能神经元、去甲肾上腺素能和肾上腺素能神经元、巨噬细胞和小胶质细胞的特异性损伤试剂等)、二抗生物素、神经元抗体、神经递质抗体、蛋白质和荧光结合物、免疫毒素对照等。